The group, Civil Human Rights Front, visited a police headquarters to apply for the permit for the weekend march.
Jimmy Sham, an activist from the Civil Human Rights Front, said the people of Hong Kong would fight "until the end" if the bill was not pulled.
Police used tear gas and pepper spray against those who came out on Wednesday to oppose the bill which protesters said could erode Hong Kong's civil liberties.
The rally marked a major escalation of the biggest political crisis in years for the semi-autonomous Chinese territory and forced the delay of legislative debate on the contentious bill.
Bonnie Leung, an activist from the Civil Human Rights Front, urged people to "stand united" and said the public still needed to "voice our opposition" to the amendment.
Members of a business association also came out to show their support to the police and the government on their handling of the protest.
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